兔肝细胞膜高密度脂蛋白受体酶联免疫检测法的研究

Study on the Enzyme linked Immunosorbent Receptor Assay of High Density Lipoprotein Receptors on Liver Plasma Membranes.

用辣根过氧化物酶标记羊抗人ａｐｏＡＩＩｇＧ， 建立了检测兔肝细胞膜ＨＤＬ受体的酶联免疫吸附检测法． 测定时，ＨＤＬ结合量按抗体配体抗配体抗体酶交联物反应制作的标准曲线确定； 膜蛋白非特异吸附则用与酶交联的抗体来源相同的同种动物血浆ＨＤＬ平行抑制试验消除． 实验测得正常家兔肝细胞膜ＨＤＬ受体Ｋｄ 值为７１７ ±１１８ ｍｇ／Ｌ， Ｂｍａｘ值为（６２２５±１４６１） ｍｇ／ｇ （ｎ＝ ７）．

Using HRP conjugated goat anti human apoAI IgG, an enzyme linked immunosorbent receptor assay was developed for measurement of high density lipoprotein (HDL) receptors on rabbit liver plasma membranes. A curve of anti apoAI IgG binding to known amounts of HDL was constructed to quantify the HDL bound. Parallel samples with 25 fold excess goat plasma HDL were assayed to detect nonspecific binding . The results showed that the K d and B max of rabbit liver plasma membrane HDL receptors were (7 17±1 18)mg/L and (622 5± 146 1)mg/L respectively ( n =7).