一个新的鼠STE20家族激酶的克隆和鉴定

Molecular Cloning and Primary Characterization of a Novel Murine STE20 like Serine/Threonine Kinase .

从鼠肝ｃＤＮＡ文库克隆了一个新的ＳＴＥ２０ 类蛋白激酶， Ｍｅｓｓ１． 其ｃＤＮＡ长１７ ｋｂ， 编码了一个４９７ 个氨基酸残基的多肽， 与人ＭＳＴ２ 具有９５％ 的氨基酸相同． Ｍｅｓｓ１ 蛋白氨基末端激酶催化区的序列与ＳＴＥ２０ 同源，其羧基末端包含了一簇丝氨酸／苏氨酸和谷氨酸丰富的序列， 被认为具有介导与ＳＨ２ 功能区结合的作用． ＭＥＳＳ１可能通过与含有ＳＨ２

A novel STE20 like protein kinase, Mess1, was cloned from a murine liver cDNA library . The 1 7 kb cDNA encodes a peptide of 497 amino acids. Mess1 is most similar to human MST2 protein kinase with an identity of 95%. A putative kinase catalytic domain, located at the amino terminus of the Mess1 protein, is homologous to that of the STE20 family. There is a serine/threonine and glutamic acid rich cluster in the carboxyl terminal region of Mess1 that is believed to mediate the binding of SH2 domains. Mess1 might involve the signal transduction on the interaction with proteins with SH2 domains.