电针和7-硝基吲唑对癫痫的效应及其与脑内一氧化氮的关系

EFFECT OF ELECTROACUPUNCTURE AND 7-NI ON PENICILLIN-INDUCED EPILEPSY AND THEIR RELATION WITH INTRAHIPPOCAMPAL NO CHANGES

为进一步探索NO在癫痫诱导和发作以及针刺抗痫中的作用，本文采用特异性nNOS阻断剂7-硝基吲唑（7－NI）观察NO合成减少时青霉素致痫的诱导和发作的变化，并使用NO敏感电极实时连续记录青霉素致痫及穴位针刺和注射7-NI时大鼠海马内NO的变化。结果表明，癫痫使发作脑电总功率剧烈升高。穴位针刺后发作明显减弱，各时间点脑电总功率与青霉素注射时有显著差别。7-NI使诱发时间缩短，阈值降低，但发作程度减轻，在各时间点脑电总功率均低于对照组。癫痫发作时海马内NO浓度明显升高，注射7-NI和穴位针刺均使NO浓度减低。结果提示，神经源性NOS可增强癫痫的发作，但延长其诱导期。穴位针刺可能通过降低nNOS水平，减少NO的生成而抑制癫痫发作。

In order to inveshgate the effect of 7-nitroindazole (7-NI) on penicillin-inducedepilepsy and the relationship between NO and anti-epileptic effect of electro-acupuncture,computerized physiological polypraph and NO-sensitive electrode with potentiostat wererespectively used to record the total power spectrum (TPS) of EEG and the concentrationof NO in rat hippocampus pretreated with electro-acupuncture or 7-NI for 30 min beforepenicillin. The results showed tha the TPS of EEG and concentration of NO inhippocampus increased greatly after the epilepsy induction. Treatment with electroacupuncthe idribited the TPS of EEG shmply (P＜0.01 ). The latency and threshold ofepilepsy induchon were decreased by 7-NI, but paroxysm abated and the wn wasiedited (P＜0.05) as compared with the conbol group. Both prebeatments with 7-NIand electroacupunthe decreased the NO concentration (P＜0.01). The above resultssuggest that the propagation of penicillin-induced seizures is fasilitated by nNOS, but withsome incnae in the latency and beshold of induction. Electroaupuncthe may iahibitseizure through decreasing nNOS transcription in hippocampus.