摘要
本实验在培养新生大鼠心肌细胞上，探讨血管紧张素Ⅱ对心肌细胞c－fosmRNA表达和蛋白质合成的影响。结果显示，血管紧张素Ⅱ能诱导c－fosmRNA的表达，增加蛋白质含量，并呈量-效关系；还能加速3H-亮氨酸的掺入速度。上述这些作用可被血管紧张素Ⅱ受体拮抗剂saralasin所阻断，提示这些作用是受体介导的。血管紧张素Ⅱ诱导c-fosmRNA的表达亦可被钙通道阻滞剂nicardipine所阻断。
The present study was to investigate the effects of angiotensin Ⅱ on c-fos mRNAexpression and protein synthesis in cultured neonatri rat myocardial cells. The resultsshowed tha angiotensin Ⅱ induced c-fos mRNA expression, increased protein content ina dose-dependent rnanner and stimulated 3H-leucine incorporation rate. All these effectswere blocked by angiotensin Ⅱ receptor antagnist snralsin. The angiotensin Ⅱ-inducedexpession of c-fos gene was also blocked by Ca2+ channel antagonist nicardipine.
出处
《生理学报》
CAS
CSCD
北大核心
1999年第5期521-526,共6页
Acta Physiologica Sinica
基金
国家自然科学基金!39170849
