尖吻蝮蛇毒NAD糖苷水解酶荧光光谱和荧光猝灭研究

Fluorescence Spectra and Quenching on NAD Glycohy drolase from the Snake (Agkistrodon acutus) Venom

70年代就有报道从蛇毒中提纯NAD糖苷水解酶(NADase,E.C.3.2.2.5)和一些生物性质方面的研究.Huang等[1]从皖南尖吻蝮蛇毒中分离得到的NADase是由两个相同亚基组成,含糖33%,等电点为7.6.刘清亮等[2]研究了NADase的ESR谱,推知Cu2+离子至少与三个氮原子配位.本文主要研究多种?..

The molecular weight of NAD glycohydrolase (NADase)(E.C.3.2.2.5) was determined accurately to be 97339.8±0.1 by LDI1700 MALDI TOF MS. Fluorescence quenching by I - and N bromosuccinimide, associating with the fluorescence spectra, suggested that Trp residues were located to a great degree in the hydrophobic areas of the inner part.