天然多糖在体外抗甲型流感病毒作用的普遍性

The anti-influenza virus activities of natural fungal polysaccharides in vitro

目的研究各种天然多糖在体外抗甲型流感病毒的作用,探索多糖未来的抗病毒药学用途。方法以云芝、虫草、灵芝、猴头菇4种天然多糖和葡聚糖为研究对象,观察天然多糖对狗肾传代细胞(MDCK)上甲型流感病毒的直接灭活作用、治疗作用、预防作用和综合阻断作用;观察天然多糖对鸡胚中甲型流感病毒的直接灭活作用。结果云芝、虫草、灵芝、猴头菇4种天然多糖对甲型流感病毒有体外灭活作用,这种作用呈剂量和时间依赖关系;毒性低,对病毒感染有一定的治疗作用,安全指数均大于14;没有明显的预防作用;有一定程度的综合阻断作用。结论在随机获取的5种多糖中,云芝等4种天然多糖具有抗甲型流感病毒的活性,说明天然多糖分子可能普遍具有抗病毒活性,如何在实际中运用多糖这一特征防治病毒感染值得进一步研究。

Objective To study the anti-influenza virus activities of a variety of natural fungal polysaccharides in vitro and explore their future pharmaceutical use.Methods Using 4 natural polysaccharides and glucan as the research object,direct inactivated,therapeutic,preventive and integrated block of polysaccharide on influenza virus A were observed on MDCK cells and chicken embryo.Results Polysaccharides have direct inactivated effect on Influenza A,it is dose-dependent and time-dependent;it is lower toxicity,have protective action,safety index 14;no remarkable preventive effect;have integrated block effect.Conclusion Natural polysaccharides can inhibit influenza A in vitro.Anti-virus activities of natural polysaccharide maybe universal and deserve further study.