近红外光谱法鉴别维C银翘片中的L-抗坏血酸

Determination of L-ascorbic acid in Vitamin C Yinqiao tablets by near-infrared diffuse reflectance spectroscopy

目的:应用近红外漫反射光谱分析技术,建立维C银翘片中L-抗坏血酸成分的快速鉴别方法。方法:近红外漫反射光谱法结合高效液相色谱法,选择抗坏血酸的近红外建模谱段,建立L-抗坏血酸成分定性鉴别模型。结果:近红外光谱法可正确判别维C银翘片中是否含有L-抗坏血酸。结论:方法快速、准确,可运用于维C银翘片中抗坏血酸及其异构体成分的快速识别。

Objective：To establish a fast identification method for the determination of L-ascorbic acid in Vitamin C Yinqiao pian by near-infrared reflectance spectroscopy（NIRS）.Methods：According to the characteric peaks of ascorbic acid in the spectrum band of NIR and the results of HPLC,the qualitative model was set up by OPUS software.Results：The near-infrared spectroscopy technology could be used to identificate whether Vitamin C Yinqiao pian contained L-ascorbic acid or not.Conclusion：The results show that the proposed method can be applied for fast detection of L-ascorbic acid and its isomer in the Vitamin C Yinqiao pian.