产过氧化氢酶菌株CE-1的筛选与鉴定

Isolation and identification of catalase-producing strain CE-1

经过含H2O2的平板初筛和摇瓶发酵复筛,从保存的水样中筛选到一株过氧化氢酶高产菌株CE-1,其所产过氧化氢酶活性和比活分别为1 356.2 U/mL和3 401 U/mg。菌株CE-1为革兰氏阴性杆状细菌,根据其形态特征、16S rRNA基因序列系统进化树分析和生理生化特性,以及属内不同种间性状差异等因素的综合分析,将菌株CE-1鉴定为气单胞属(Aeromonas)细菌。

Catalase-producing strain CE-1 was isolated from water samples using H2O2-containing plate screening and activity assay of the shake flask culture method.The values of the activity and specific activity were 1356.2 U/mL and 3401U/mg,respectively.CE-1 was Gram negative and rod in shape.Based on the comprehensive analysis of morphological and physiological characteristics,the 16S rDNA phylogenetic tree,and the differences among different species of Aeromonas,strain CE-1 was identified to be Aeromonas.