摘要
目的观察低浓度Aβ1-42单体/寡聚体对胆碱能SN56细胞存活率的影响以及不同浓度CORM-2的作用?方法将相同细胞浓度的SN56细胞培养在96孔细胞培养板中并将细胞分为对照组、Aβ1-42组、Aβ1-42+CORM-250μM组及A[3142+CORM-2100μM组:Aβ1-42组三列细胞分别在10nM、100nM及1μMAβ1-42单体/寡聚体的环境中培养.Aβ1-42+CORM-250μM组和A13。+CORM-2100μM组细胞除每孔分别含50μM及100μMCORM-2外,其余培养条件与Aβ1-42组相同。对照组不加任何处理因素。培养3d后,用MTF法比较不同组别细胞存活率。结果Aβ1-42组细胞存活率分别为(79.73±0.94)%、(67.99±0.79)%、(60.42±0.62)%,随Aβ1-42浓度增加而下降。Aβ1-42+CORM-250μM/100μM组细胞存活率分别为(75.15±0.096)%、(63.20±0.17)%、(55.33±0.19)%;(73.20±0.27)%、(64.34±0.11)%、(54,17±0.12)%,均低于单纯Aβ1-42组;并且不同CORM-2浓度之间,降低细胞存活率的作用存在差异。结论低浓度Aβ1-42单体/寡聚体可降低SN56细胞的存活车,并且浓度越高,效果越明显;不同浓度CORM-2均可使SN56细胞的存活率下降.且作用强度存在差异。
Objective 'ro observe the effect of low concentration Aβ1-42 nmnomer/oligomers and CORM-2 in different concentration on livabilitv of SN56 cells. Methods SN56 cells were cuhured in the 96-well plate with uniform concentration, and were divided into control group, Aβ1-42 group, Aβ1-42 + CORM-2 50μM group, and Aβ1-42+ CORM-2 100 o.M group. Three lines of cells in Aβ1-42, group were cuhnred in the surroundings of 10 nM, 100nM and 1 μMAβ1-42monomer/oligomers, respectively. Aβ1-42 + CORM-2 50μM group and Aβ1-42 + CORM-2 100μM group had the same cuhure condition as group Aβ1-42,except contain 50μM, and 100μM CORM-2, respectively. Control group didnt have any effect factor. Three days later,the fivabilily of different groups was compared with MTT method. Results The livability of group Aβ1-42 with the increasing concentration of Aβ1-42 was ( 79.73 ± 0.94 ) % , ( 67.99 ± 0.79 ) % , ( 60.42 ± 0.62 ) % , respectively. The higher the concentration of Aβ1-42 was,the lower the livability of SN56 cell was. The livability of group Aβ1-42 + CORM-2 501.zM/100txM was(75.15 ±0.096)% ,(63. 20 ± 0. 17)%, (55. 33 ±0. 19)% ; (73.20 ±0. 27)%, (64. 34 ±0. 11 )%, (54. 17± 0. 12)% , respectively. Both were Dower than group Aβ1-42 And different CORM-2 concentration had discrepancy in the ability of decreasing the cell livability. Conclusion Low concentration of Aβ1-42 can reduce the livability of SN56 cells, and higher concentration has more significant effect; CORM-2 in different concentration both can decrease the livahility of SN56 cells,and there is a discrepancy in the intensity.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2010年第12期1096-1098,共3页
Chinese Journal of Behavioral Medicine and Brain Science
基金
基金项目:河北省国际科技合作行划项目(09396427D)