蜈蚣藻抗肿瘤活性及其化学成分研究

Study on the anticancer activities and chemical constituents of the Grateloupia filicina C.Ag.

目的研究蜈蚣藻不同提取部位的体外抗肿瘤活性及其物质基础。方法蜈蚣藻经乙醇提取后,用石油醚、醋酸乙酯和正丁醇依次对其进行分段萃取。采用MTT法测定各提取部位对体外培养人肺腺癌细胞系A549、肝癌细胞系HepG2、宫颈癌细胞系HeLa、乳腺癌细胞系MDA-MB-468和MCF-7的抑制作用;应用硅胶、反相硅胶ODS-A、Sephadex LH-20柱色谱对其活性部位进行分离纯化,并确定化合物结构。结果蜈蚣藻的石油醚萃取部位对MDA-MB-468乳腺癌细胞、HepG2肝癌细胞、A549肺癌细胞有明显抑制作用。并从该部位分离得到6个化合物,分别为十六烷酸(1)、胆甾醇(2)、大黄素(3)、24-乙酰泽泻醇A(4)、25-脱水泽泻醇A(5)和泽泻醇A(6)。6个化合物均为首次从该海藻中分离得到,其中,化合物3～6为首次从该属海藻中分离得到。结论蜈蚣藻的石油醚部位是其主要的抗肿瘤活性部位,这可能与从该部位分离得到的6个化合物相关。

Objective To investigate in vitro anticancer activities of different extraction parts from Grateloupia filicina C.Ag.and the chemical constituents of therein contributable to anticancer activity. Methods The extraction from Grateloupia filicina C.Ag.with ethanol and then from the ethanol extraction part in turn with petroleum ether,ethyl acetate and n-butanol was conducted.The inhibitory effects of these extraction parts on A 549,HepG2,HeLa、MDA-MB-468 and MCF-7 cells were measured by MTT assay.Compounds of anti-tumor active part were isolated by silica gel,ODS-A,Sepha- dex LH-20 column chromatographies.Their structures were identified hy spectral methods.Results The petroleum ether part showed significant inhibition of A549,HepG2,HeLa,MDA-MB-468 cell lines.Six compounds were isolated from the petroleum ether part.They were identified to be palmitic acid（1）,cholesterol（2）,emodin（3）.alisol A 24-acetate（4）,25-anhydroalisol A（5）,alisol A（6）. Compounds 1～6 were all isolated from Grateloupia filicina C.Ag.for the first time,while compounds 3～6 were first reported from this Grateloupia C.Ag..Conclusion The petroleum ether part of Grateloupia filicina is the main anticancer active part,which could be associtated with the isolated 6 compounds therein.