利用GC-MS测定不同甲酯化条件下沙蜇脂肪酸组成

Analysis of fatty acids in Stomolophus meleagris prepared with different methyl esterification methods by GC-MS

目的测定山东青岛海域成熟沙蜇伞部、头部、触手、里子和生殖腺5个部位的脂肪酸组成和含量。方法提取沙蜇不同部位的脂肪酸,通过酸催化、皂化催化和重氮甲烷3种甲酯化方法,运用GC-MS进行测定和分析。结果沙蜇含44种脂肪酸,其中含有丰富的多不饱和脂肪酸DHA 3.13%～13.97%、EPA 1.13%～28.81%和AA 3.62%～27.53%,饱和脂肪酸中奇数碳原子和异构脂肪酸的种类和含量较多,达到7.63%～41.67%。结论 3种甲酯化方法测出的相同部位脂肪酸种类和含量有差异,不同部位脂肪酸的种类和含量也有差异。沙蜇的脂肪酸种类齐全,营养价值较高。

Objective The fatty acids composition in five different parts（umbrella、oral arms、tentacle、sarcenet and gonads） of full-grown Stomolophus meleagris from the Yellow Sea was investigated.Methods The fatty acids were extracted and their components were analyzed by gas chromatography/mass spectrometry （GC/MS）,using three methods of methyl esterification including acid-catalyzed method,basecatalyzed method and Diazo（trimethylsilyl） methane method.Results The results showed that there were 44 kinds of fatty acids in Stomolophus meleagris,percentages of polyunsaturated fatty acids（PUFA） were high,（docosahexaenoic acid（DHA） 3.13%～13.97%,eicosapentaenoic acid（EPA） 1.13%～28.81%and eicosatetraenoic acid（AA） 3.62%～27.53%）.The compositions and percentages of odd and branched-chain fatty acids in the saturated fatty acids were high,7.63%～41.67%.Conclusion The compositions and percentages of the fatty acids in the same part using three methods of methyl esterification are different,the compositions and percentages of fatty acids in different parts are also different. The fatty acids of Stomolophus meleagris have high nutritive value.