层流剪切力对脐血CD34^+细胞向各系分化的影响

Effects of Laminar Shear Stress on the Differentiation of Cord Blood CD34^+ Cells

为了研究层流剪切力对造血干细胞体外扩增及向各系造血祖细胞分化的影响,考察了在3组不同大小层流剪切力0、0.03、0.06 Pa的作用下,CD34+细胞为起始细胞的造血干/祖细胞体外扩增和向各系祖细胞分化的情况.结果表明:3组总细胞、CD34+细胞及CD34+38-细胞的扩增无显著性差异;0.03 Pa层流剪切力使造血干细胞更易于向红系祖细胞系分化;0.06 Pa层流剪切力使其更易于向粒-巨噬祖细胞系分化;无层流剪切力作用下造血干细胞更易于向混合系祖细胞系分化.

In order to study the effects of laminar shear stress on the ex vivo expansion and differentiation of cord blood hematopoietic stem cells,three laminar shear stress of 0,0.03,0.06Pa are mainly investigated.The conclusion is that the laminar shear stress has no significant difference in the ex vivo expansion of total cells,CD34^＋ cells and CD34^＋38^-cells;When cultivated under 0.03Pa LSS,the HSC tends to differentiate from BFU-E.When cultivated under 0.06Pa LSS,the HSC tends to differentiate from CFU-GM,and when cultivated under 0Pa,the HSC tends to differentiate from CFU-GEMM.