糖苷酶对橙皮苷和柚皮苷生物利用率的影响

EFFECT OF GLYCOSIDE HYDROLASE ON BIOAVAILABILITY OF HESPERIDIN AND NARINGIN

目的研究糖苷酶处理橙皮苷和柚皮苷后,糖苷结构中的糖配是否基影响了其生物利用率。方法通过大鼠一次性灌胃橙皮苷81.9μmol(Ⅰ)和柚皮苷86.2μmol(Ⅳ)、经酶处理的橙皮苷81.9μmol(Ⅱ)和柚皮苷86.2μmol(Ⅴ)以及它们的苷元橙皮素81.6μmol(Ⅲ)和柚皮素85.8μmol(Ⅵ),再分别检测大鼠血清中经β-葡萄糖醛酸酶水解的总的橙皮素和柚皮素的浓度。结果第Ⅱ组的最大浓度值Cmax分别比第Ⅰ组和第Ⅲ组高70%和188%;第Ⅱ组到达最大浓度时的时间Tmax比第Ⅰ组快4 h,比第Ⅲ组慢4 h;浓度-时间曲线以下围成的面积AUC值第Ⅱ组分别比第Ⅰ组和第Ⅲ组高14%和55.4%。第Ⅴ组的Cmax分别比第Ⅳ组高出1倍多,比第Ⅵ组高25.7%;第Ⅴ组Tmax分别比第Ⅳ组快5 h,比第Ⅵ组快1.67 h;第Ⅵ组AUC值的略高于第Ⅴ组,第Ⅴ组的比第Ⅳ组的要高出34.5%。结论经过酶处理的橙皮苷和柚皮苷的生物利用率比原黄酮提高,吸收速度也加快。

Objective To study whether the effect of glycoside hydrolase on bioavailability of hesperidin and naringin was through their sugar moieties.Method Male rats were administered i.g once with natural hesperidin 81.9 μmol（groupⅠ） and naringin 86.2 μmol（groupⅣ）,hesperidinase treated hesperidin 81.9 μmol（groupⅡ）and naringinase treated naringin 86.2 μmol（groupⅤ） as well as hesperetin 81.6 μmol（groupⅢ） and naringenin 85.8 μmol（groupⅥ） respectively.Afterwards hesperetin and naringenin hydrolyzed with β-glucuronidase in plasma were detected.Results The peak plasma concentrations（Cmax） of hesperetin in Group Ⅱ were 70% and 188% higher than that in GroupⅠand GroupⅢ respectively.The time to reach the maxium concentration（Tmax） of plasma hesperetin in Group Ⅱ was 4h faster and 4h slower than that in GroupⅠ and Group Ⅲ respectively.The area under the curve（AUC） of plasma hesperetin in Group Ⅱ was 14% and 55.4% more than that in GroupⅠ and Group Ⅲ respectively.Similarly,Cmax of plasma naringenin in Group Ⅴ was one-fold and 25.7% higher than that in Group Ⅳ,and Group Ⅵrespectively.Tmax of plasma naringenin in GroupⅤ was 5h and 1.67 h faster than that in Group Ⅳ and GroupⅥ respectively,The AUC of plasma naringenin in Group Ⅵ was slightly higher than GroupⅤ,while that in Group Ⅴ was 34.5% higher than Group Ⅳ.Conclusion The bioavailability of hesperidin and naringin was improved by enzymatic treatment,thus absorbed easily.