食用百合试管苗缓慢生长保存的研究

Studies on Edible Lily in vitro Conservation Technique by Slow-growth Method

为了建立食用百合种质资源缓慢生长保存体系,将扩繁培养后得到的试管苗接种到12种不同培养基中,10℃和25℃下分别保存6个月,观察不同处理试管苗生长情况。结果表明:低浓度甘露醇对百合试管苗生长的抑制效果差,而高浓度甘露醇严重影响其生长势,缓慢生长保存的最适浓度为20g·L-1;蔗糖对百合试管苗的生长有抑制作用,适宜浓度为60g·L-1;20g·L-1甘露醇+60g·L-1蔗糖处理的百合试管苗存活率均达100%,且有鳞茎形成,保存效果最好。10℃保存的试管苗生长缓慢,有利于鳞茎形成,保存至6个月时不需要继代培养;25℃保存试管苗的鳞茎形成率低,培养基损失量高,必须继代才能继续保存。

In order to establish conservation system for edible lily slow-growth germplasm resources,the test-tube plantlets gained from micro-propagation were inoculated in 12 different culture media and conserved under 10 ℃ and 25 ℃ for 6 months,respectively,so as to observe the growth of test-tube plantlets under different treatments.The results showed that the low mannitol concentration has poor control affect on the growth of test-tube plantlets,while the high concentration severely affect the plantlets growth.The suitable mannitol concentration was 20 g·L-1 for conserving edible lily plantlets with slow-growth.Sucrose has restrain effect on the growth of test-tube plantlet,60 g·L-1 sucrose is the suitable media.20 g·L-1 mannitol＋60 g·L-1 sucrose is the best treatment for slow growth conservation of edible lily test-tube plantlets.Their survival rate can reach 100 %,and the rate of bulb formation is high.10 ℃ can keep the plantlet grow slowly and promote the bulb formation for 6 months conservation without subculture,while under 25 ℃ the rate of bulb formation is reduced,and the medium loss is serious.The plantlets must be preserved with subculture.