牛流行热病毒结构糖蛋白(G)基因的克隆及鉴定

Clone and identification of G Protein Gene of Bovine Ephemeral Fever Rhabdovirus

牛流行热是由病毒引起的牛和水牛的急性热性传染病。其G蛋白为81kDa的结构糖蛋白，它位于病毒表面并含有中和抗原位点，可使牛产生免疫保护。本研究利用RT-PCR法从牛流热病毒北京血毒JB76H总RNA中，扩增并克隆了其G蛋白基因（1.9Kb）。限制性内切酶酶切分析结果显示，与澳大利亚分离株BB7721差异较大。脱氧核糖核苷酸序列分析表明，该基因与澳大利亚分离株BB7721同源性高达91％。

Bovine ephemeral fever virus(BEFV) is an arthropod-borne rhabdovirus which causes a disabling febrile infection of cattle and water buffalo. The disease is common in tropical and subtropical regions of Africa, Asia, Australia and the Middle-east and is of major economic importance to dairy and grazing industries. The surfare glycoprotein G is the major neutralizing and protective antigen of BEFV. In this report we cloned the gene of C protein from the genome RNA of BEFV by RT-PCR. The nucleotide Sequence of this gene showed 91% homology compared with Australia isolate BB7721.