HPLC法测定棉团铁线莲中齐墩果酸的含量

Determination of Oleanolic Acid in Roots and Stem-Leaf of Clematis hexapetala by HPLC

[目的]采用HPLC法测定棉团铁线莲(Clematis hexapetala)根及茎叶中齐墩果酸含量,为进一步开发棉团铁线莲药用资源提供理论依据。[方法]色谱柱为YW-C18柱(4.6 mm×150.0 mm,5μm);流动相为甲醇-水(V∶V=90∶10);检测波长为220 nm;进样量为10μl;流速为1.0 ml/min;柱温为25℃。[结果]棉团铁线莲根中齐墩果酸的含量为0.175 2%,茎叶中齐墩果酸的含量为0.096 3%。[结论]该方法简单、快速、准确,可用于棉团铁线莲中齐墩果酸的含量测定;同时该研究为评价棉团铁线莲茎叶的药用价值提供了参考,为进一步开发棉团铁线莲药用资源和质量控制提供了科学依据。

[Objective] To determine the content of oleanolic acid in roots and stem-leaf of Clematis hexapetala,so as to provide theoretical evidence for the further development of Clematis hexapetala resources.[Method] HPLC conditions were as follows： YW-C18 column（4.6 mm×150 mm,5 μm）,mobile phase of methanol-water（V∶V=90∶10）,detection wavelength of 220 nm,sampling amount of 10 μl,flow rate of 1.0 ml/min and column temperature of 25 ℃.[Result] The content of oleanolic acid in roots and stem-leaf of Clematis hexapetala were 0.175 2% and was 0.096 3% respectively.[Conclusion] Simple and rapid in operations and accurate in determination,the method established in this study is favorable for the determination of oleanolic acid in Clematis hexapetala,This case provides reference for the assessment of the medicinal value of stems and leaves from Clematis hexapetala,and offers scientific basis for the further development and quality control of Clematis hexapetala resources.