分光光度法测定伏牛山荷叶中微量元素锌

Determination of Zn in Funiushan FOLIUM NELUMBINIS by Ultraviolet Spectrophotometry

[目的]测定荷叶（FOLIUMNELUMBINIS）中微量元素锌的含量,为荷叶在医药及食品工业的深度开发利用提供科学依据。[方法]采用直接分光光度法,以1-（2-吡啶偶氮）-2-萘酚（PAN）为显色剂、锌标准液为对照品,在562 nm波长处测定吸光度。[结果]Zn（Ⅱ）与PAN形成红色的络合物,在0.48~5.03μg/ml范围内,吸光度值与含量线性关系良好（r=0.999 9）;平均回收率为99.8%,RSD为0.34%。伏牛山荷叶中锌元素的平均含量为87.44μg/g,RSD为1.02%。[结论]该方法简便、准确、重现性好,稳定性高,可用于荷叶中微量元素锌含量的测定。

[Objective] The aim was to determine the Zn content in FOLIUM NELUMBINIS so as to provide scientific basis for the deep development and utilization of FOLIUM NELUMBINIS in pharmaceutical and food industries.[Method] Direct spectrophotometry method was used for the determination of Zn in lotus leaf with PAN as the color reagent and zinc standard solution as reference at the 562 nm wavelength.[Result] Zn（Ⅱ） and PAN could form red complex,the absorbance had good linear relationship with the content within the range of 0.48-5.03 μg/ml（r=0.999 9）,and an average recovery was 99.8%,RSD was 0.34%.The content of Zn in lotus leaf was 87.44 μg/g and RSD was 1.02%.[Conclusion] The method was simple and accurate with good reproducibility and high stability,which could be used for the determination of Zn in FOLIUM NELUMBINIS.