“青海444”燕麦中皂苷提取工艺研究

Study on the Technology of Extraction in "Qinghai 444" Oat Saponin

[目的]研究利用索氏浸提法提取燕麦中皂苷的工艺条件。[方法]以乙醇?正丁醇为溶剂,以皂苷Re为标准品,采用分光光度法定量分析燕麦提取液中总皂苷含量,利用正交试验设计研究料液比、乙醇浓度、浸提温度对提取率的影响。[结果]各因素对皂苷提取率影响的主次顺序依次为:乙醇浓度>料液比>浸提温度;最佳提取条件:料液比为1∶7,乙醇浓度70%,浸提温度60℃;在此条件下,提取率为2.504%。[结论]该方法操作方便,简单易行,具有推广应用价值。

[Objective] The technology condition of extracting saponin from oat by the method of soxhlet extraction was studied.[Method] The saponin were extracted by ethanol-butanol as the solvent from oat and the content was determined by spectrophotometry.Orthogonal experiment was used to study the extraction technology of saponin in oat.Effects of solid-liquid ratio,ethanol concentration,extraction temperature on extraction rate of saponin were studied.[Result] The optimal extracting conditions of oat saponin were established as follows： solid-liquid ratio 1∶7,ethanol concentration 70%,extraction temperature 60 ℃;under these conditions,the extraction ratio was 2.504%;the influence order of extraction rate was ethanol concentrationsolid-liquid ratioextraction temperature.[Conclusion] The method had many advantages listed below： convenient in operation,easy access,and possessed of popularization and application value.