黄荆子乙酸乙酯提取物诱导人宫颈癌Hela细胞凋亡

Acetoacetate Extracte of Vitex Negundo Seed Induced Apoptosis in Hela Cell Line

[目的]探讨黄荆子乙酸乙酯提取物(EVn-50)体外诱导人宫颈癌Hela细胞的凋亡作用。[方法]体外培养Hela细胞,不同浓度EVn-50(1.0、10.0、100.0μg/ml)作用于Hela细胞。采用PI染色FCM检测EVn-50诱导Hela细胞的凋亡作用;采用间接免疫荧光标记FCM检测凋亡相关蛋白Bcl-2、Bax、Caspase-3的表达。[结果]EVn-501.0、10.0、100.0μg/ml处理Hela细胞48h后,细胞的凋亡率分别为1.06%、8.80%及20.90%,呈浓度依赖性(P<0.05)。不同浓度EVn-50作用Hela细胞48h,细胞中Bax、Caspase-3蛋白表达逐渐升高,Bcl-2蛋白表达逐渐降低(P<0.05)。[结论]EVn-50具有诱导Hela细胞凋亡作用,其作用可能与改变凋亡相关蛋白Bax、Caspase-3及Bcl-2的表达有关。

[Purpose] To investigate the effect of acetoacetate extracte of Vitex negundo seed（EVn50） induced apoptosis in Hela cell line.[Methods] In vitro,Hela cells were incubated with EVn-50 at different dose（1.0,10.0 and 100.0μg/ml）.The effect of EVn-50 induced apoptosis of Hela cell was verified by flow cytometry（FCM） with PI staining.The expression of Bcl-2,Bax and Caspase-3 protein in Hela cells were detected by FCM indirect immunofluorescent technique.[Results] After 48h treatment with EVn-50 at 1.0,10.0 and 100.0μg/ml,the apoptosis rates of Hela cells were 1.06%,8.80% and 20.90% respectively（P0.05）,in a dose-dependent manner.In addition,the expression of Bax and Caspase-3 was upregulated,while the expression of Bcl-2 was downregulated with the increasing doses of EVn-50（P0.05）.[Conclusion] EVn-50 can induce apoptosis in Hela cells.The mechanism might relate to the expression of Bax,Caspase-3 and Bcl-2 protein.