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人CYP19基因启动子荧光素酶报告基因载体的构建 被引量：1

Construction of Luciferase Report Gene Vector Containing Human CYP19 Promotor
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摘要 [目的]克隆人CYP19基因启动子PⅠ.3(-345～-234bp)、PⅡ(-517～-278bp)和PⅠ.7(-299～+81bp)片段,构建启动子荧光素酶报告基因载体。[方法]采用生物合成PⅠ.3(-345～-234bp)、PⅡ(-517～-278bp)和PⅠ.7(-299～+81bp)区域基因片段,通过HindⅢ和KpnⅠ酶切连接到pGL3-Basic载体上。[结果]测序验证pGL3-Basic-PⅠ.3,pGL3-Basic-PⅡ和pGL3-Basic-PⅠ.7构建成功。[结论]pGL3-Basic-PⅠ.3,pGL3-Basic-PⅡ和pGL3-Basic-PⅠ.7荧光素酶报告基因载体的成功构建,为进一步研究CYP19基因组织特异性表达奠定基础。 [Purpose] To construct luciferase report gene vector containing human CYP19 promotor PⅠ.3（-345--234bp）,PⅡ（-517--278bp）and PⅠ.7（-299-＋81bp）.[Methods] PⅠ.3（-345-234bp）,PⅡ（-517--278bp）and PⅠ.7（-299-＋81bp） were amplified by biosynthesis,and inserted into the luciferase report gene pGL3-Basic vector with restriction endonuclease HindⅢ and Kpn Ⅰ.[Results] pGL3-Basic-PⅠ.3,pGL3-Basic-PⅡ and pGL3-Basic-PⅠ.7 were confirmed by DNA sequencing.[Conclusion] The human CYP19 promotor luciferase reporter gene vectors are constructed successfully,which establishes the base for the further research of CYP19 tissue-specific expression mechanism.
作者 邵喜英 陈占红 黄健 雷蕾 冯建国 苏丹 王晓稼
机构地区 浙江省肿瘤医院 浙江省肿瘤研究所
出处 《肿瘤学杂志》 CAS 2010年第11期873-876,共4页 Journal of Chinese Oncology
基金 浙江省自然科学基金(Y206856) 浙江省医药卫生优秀青年科技人才专项基金(2005QN002) 浙江省医药卫生科学研究基金资助项目(2007B021)
关键词 CYP19基因 启动子 荧光素酶 CYP19 gene promotor luciferase
分类号 R730.23 [医药卫生—肿瘤]
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参考文献8

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同被引文献29

  • 1王安平,孙怀昌,王建业,王永娟,袁维峰.表达绿色荧光蛋白报告基因重组禽腺联病毒载体系统的构建与鉴定[J].病毒学报,2007,23(4):292-297. 被引量：7
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肿瘤学杂志
2010年 第11期

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