摘要
目的评价氯沙坦联合小剂量叶酸对自发性高血压大鼠(SHR)血液流变学和血管结构的影响。方法 30只12周龄SHR,随机分为3组(n=10),空白对照组(SHR-C),氯沙坦组(SHR-L:50 mg.kg·1.d-1),合用组(SHR-L+Y:氯沙坦25 mg.kg·1.d-1+叶酸0.4 mg.kg·1.d-1),另选10只同龄Wistar大鼠作为正常对照组(WKY-C)。连续给药12周后,测定血液流变学指标,采用单因素方差分析,以LSD法进行组间比较。肠系膜血管组织切片用HE染色,主动脉测定血管壁厚度和管腔面积,计算厚度和管腔面积。结果 SHR-L+Y组与SHR-C相比,全血黏滞度各指标均下降,P<0.05;SHR-L+Y组与SHR-L相比,全血黏滞度无明显差别,P>0.05。血浆黏滞度,各组间差别不显著,P>0.05。血管组织学观察显示:SHR-L+Y组肠系膜动脉无增厚,内皮细胞层完整,内弹性膜结构清晰,主动脉中膜面积和管腔面积比与SHR-C组相比有统计学差异,P<0.05。结论低剂量的氯沙坦和叶酸能有效的改善血液流变学,保护血管内皮,防止血管重构。
OBJECTIVE To investigate the efficacy of losartan-Folic acid in hemorheology level and vascular endothelium.METHODS Thirty spontaneously hypertensive rats(SHR),aged 12 weeks,enrolled for a randomized controlled trial.Subjects were randomly assigned to the following three groups(n=10):controlled group(SHR-C),losartan group(SHR-L:50 mg·kg-1·d-1),losartan-Folic group(SHR-L+Y: losartan 25 mg·kg-1·d-1+Folic acid 0.4 mg·kg-1·d-1),another 10 of the same age Wistar Rats assigned for normal controlled group(WKY-C).Hemorheology was examined after oral administration for 12 weeks.The data was performed analysis of variance and LSD-t.Vessels of mesentery and aorta were stained by HE.The thickness and area of aorta was measured and analyzed by computer.RESULTS The viscosity of whole blood was decreased significantly in SHR-L+Y group compared with SHR-C,P0.05.There are no obvious difference of hemorheology between SHR-L+Y group and SHR-L group,P0.05.Effects on plasma viscosity was not significantly different among the four groups.For morphological remodeling in group SHR-L+Y,there are no thickness increased in mesenteric artery and endothelial wall is integrated,aortic tunica intima is smooth,the media cross-sectional area and vessel area of SHR-L+Y compared with group SHR-C was not significantly different,P0.05.CONCLUSION Losartan-Folic acid could be used to decrease the viscosity,protect vascular endothelium and reversing vascular reconstruction.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2010年第12期1077-1080,共4页
Chinese Journal of Modern Applied Pharmacy
关键词
氯沙坦
叶酸
自发性高血压大鼠
血液流变学
血管
losartan
Folic acid
spontaneously hypertensive rats
hemorheology
vascular