初探异叶败酱提取物对鸡胚翅芽发生中基因表达模式的作用

Effects of Extracts from Patrinia heterophylla on Gene Expression Patterns during Morphogenesis of Chicken Limb Buds in vivo

目的:利用鸡胚翅芽模型检测异叶败酱提取物对胚胎发育过程中基因表达模式的调控作用。方法:将带有异叶败酱提取物的丙烯酰胺念珠植入鸡胚翅芽,进行原位杂交,观察异叶败酱提取物对基因Myf5、Myod和PC-NA表达的影响。结果:异叶败酱提取物(200 mg/mL)能下调在翅芽发育过程中基因Myf5、Myod和PCNA表达。结论:异叶败酱提取物能抑制细胞分化和增殖。

Objective：To study the effects of the extracts from Patrinia heterophylla on gene expression patterns during morphogenesis of chicken limb buds in vivo.Methods：Implanted a bead into an chicken embryo,which was soaked in the extracts from Patrinia heterophylla.Detected the extracts-induced morphogenesis changes（Myf5,Myod and PCNA）.Results：The extracts from Patrinia heterophylla（200 mg/mL） could affect limb bud development,reduce gene expression of Myf5,MyoD and PCNA.Conclusion：The extracts from Patrinia heterophylla can inhibit cell differentiation and proliferation.