大孔吸附树脂富集纯化鸡血藤总黄酮的研究

Enrichment of Total Flavonoids from Spatholobus suberectus by Macroporous Adsorption Resin

目的:研究不同类型吸附树脂对鸡血藤中黄酮的吸附特性。方法:测试了6种树脂对鸡血藤总黄酮的吸附率、吸附速率、解吸率和产物得率及其黄酮含量。结果:HPD 400树脂对鸡血藤总黄酮吸附量大、吸附快、解吸容易,吸附后产物黄酮含量高。结论:HPD 400、AB-8弱极性树脂是性能良好的鸡血藤总黄酮吸附剂,可用于从鸡血藤提取物中富集总黄酮,制备含量达50%以上的总黄酮,为鸡血藤中总黄酮的开发提供新工艺。

Objective：To screen different macroporous adsorption resins for isolation and purification of flavonoids from Spatholobus suberectus.Methods：6 kinds of macroporous adsorption resins were assayed for their adsorbability and deadsorbability of total flavonoids from Spatholobus suberectus.Results：HPD 400 resin was found with good adsorbability and deadsorbability.Conclusion：HPD 400 resin and AB-8 resins can be used for enriching high contents of total flavonoids from Spatholobus suberectus,and more than 50% of flavonoids were enriched from the extract of Spatholobus suberectus.