HPLC法同时测定麻仁丸中芍药苷、柚皮苷及橙皮苷的含量

Simultaneous Determination of Paeoniflorin,Naringin and Hesperidin in Maren Pills Processed by HPLC

目的:增加麻仁丸的含量测定方法,建立同时检测芍药苷,柚皮苷及橙皮苷的测定方法。方法:采用C18色谱柱;水-乙腈为流动相,进行梯度洗脱;流速1.0mL·min-1;检测波长230nm。结果:线性范围:芍药苷0.03075～0.2050g·L-1(r2=0.9997),柚皮苷0.1148～0.7650g·L-1,r2=0.9997,橙皮苷0.01275～0.08500g·L-1(r2=0.9996);平均回收率:芍药苷99.4%,RSD=0.9%(n=6),柚皮苷99.7%,RSD=1.2%(n=6),橙皮苷98.6%,RSD=2.0%(n=6)。结论:该方法操作简便、准确,重复性好,可用于麻仁丸的质量控制。

Objective：The method of determination of the paeoniflorin,naringin and hesperidin in Maren Pills processed by HPLC was established. Methods：The column was C18 Column, the mobile phase was water acetonitrile for gradient elution, the flow rate was 1.0 mL·min-1 and the detection wavelength was 230 nm. Results：The linear range of Paeoniflorin was 0.030 75～0.205 0 g·L-1 （r2=0.999 7）, the average recovery was 99.4%, RSD=0.9%（n=6）.The linear range of naringin was 0.012 75～0.085 00 g·L-1 （r2=0.999 7）, the average recovery was 99.7%,RSD=1.2%（n=6）.The linear range of hesperidin was 0.012 75～0.085 00 g·L-1 （r2=0.999 6）, the average recovery was 98.6%, RSD=2.0%（n=6）.Conclusions： This method is simple,rapid and accurate with good reproducibility,it is suitable for the quality control of Maren Pills processed.