摘要
目的:研究脐带血间充质干细胞体外分离、培养条件,探讨脐带血间充质干细胞作为细胞治疗种子细胞的可行性。方法:采用不同的实验方法对36例脐血间充质干细胞分离、培养,并与11份骨髓间充质进行对比,探索脐血间充质干细胞的最适培养条件。结果:采用甲基纤维素分离的间接法显著提高了脐血间充质干细胞分离培养的成功率;增加单个核细胞的接种量和适当的血清浓度促进了脐血间充质干细胞的成功培养。结论:脐血间充质干细胞能在体外分离纯化,但培养成功率明显低于骨髓,受多种因素影响。
Objective：To investigate the optimal method for the isolation, culture and identification of human umbilical cord blood mesenchymal stem cells（MSCs）in vitro,and to observe the feasibility of using MSCs as seed cells for cell therapy.Methods：MSCs were isolated from normal human umbilical cord blood,cultured in vitro,purified and contrast with 11 Bone Marrow Mesenchymal Stem Cells（BMMSCs）. Results：Methylcellulose method could increase success frequences of culturing MSCs from UCB.Higher density and proper FCS were favorable for MSCs culture of UCB. Conclusion：The navel blood mesenchymal stem cell can in vitro separation purification, but the raise success ratio is lower than the marrow obviously,many kinds of factor influences the result.
出处
《中国医药导刊》
2010年第12期2124-2127,共4页
Chinese Journal of Medicinal Guide
关键词
间充质干细胞
脐血
骨髓
体外培养
Mesenchymal stem cells
Umbilical cord blood
Bone marrow
Isolation and culture in vitro
