HBV感染者血清表面抗原大蛋白,DANE颗粒,HBV-DNA与PreS1蛋白检测分析

Detection and Analysis of Serum Hepatitis B Virus Large Surface Protein,DANE Particle, HBV-DNA and PreS1-Ag to Hepatitis B Virus Patients

目的 检测HBV感染者血清中乙肝表面抗原大蛋白（LHBS）,DANE颗粒,HBV-DNA和PreS1蛋白,探讨LHBS在临床诊治中的价值.方法 对1 042例HBV感染者采用酶联免疫吸附试验检测乙肝病毒HBeAg,LHBS,DANE颗粒和PreS1蛋白,并采用定性PCR检测HBV-DNA.结果 245例HBeAg（＋）的HBV感染者中,LHBS阳性率（96.33%）,DANE颗粒阳性率（90.20%）,分别与HBV-DNA阳性率（92.65%）比较,差异无统计学显著性意义（P＞0.05）,三者均明显高于PreS1阳性率（66.53%）,差异均有统计学显著性意义（P＜0.01）;797例HBeAg（-）的HBV感染者中,LHBS阳性率（78.17%）高于DANE颗粒阳性率（56.46%）,HBV-DNA阳性率（33.12%）和PreS1阳性率（17.44%）,依次比较差异均有统计学显著性意义（P＜0.01）.结论 LHBS是判断HBV感染者体内病毒复制程度的可靠指标,在HBeAg（-）的HBV感染者中,LHBS有指导治疗、预后判断的作用;DANE颗粒亦是判断病毒复制程度的可靠的新指标.LHBS和DANE可用于暂无条件开展PCR实验的的基层医疗单位,代替HBV-DNA检测.

Objective To evaluate the significance of the serum Hepatitis B virus large surface protein detection to HBV patients. Methods LHBS,DANE particle and PreS1-Ag were detected with ELISA while HBV-DNA with PCR in 1 042 serum samples collected from HBV patients. Results In 245 sample from HBeAg（＋） serum,there was no significant difference between the positive rate of LHBS （96.33 % ）, DANE particle （90.20% ） and HBV-DNA （92.65 % ）, （separately, P〉0. 05） ;PreS1-Ag （66. 53% ） was lower than others significantly（separately,P〈0.01）. In 797 samples from HBeAg（-） serum, the positive rate of LHBS （78.17%） was significantly higher than DANE particle （56.46%）, HBV-DNA （33.12%） and PreS1-Ag （17.44%）, （separately,P〈0. 01）. Conclusion It is suggested that LHBS may be served as reliable marker of reflecting HBV replication. LHBS is valuable to monitor the treatment and diagnosis of the patients with HBeAg（- ） chronic hepatitis B. DANE particle also could reflect HBV replication. LHBS and DANE particle could be used by the basic medical infirmary to replace PCR which is expensive and complicated.