耐甲氧西林金黄色葡萄球菌的两种同源性分析方法的比较

Analysis Methicillin Resistant Staphylococcus of Sequence Homology in Two Methods

目的 比较使用药物敏感试验的表型研究方法与随机扩增多态DNA（RAPD）染色体分析方法对耐甲氧西林金黄色葡萄球菌（MRSA）的同源性分析,探讨使用表型方法研究细菌同源性的可行性.方法 采用WHO推荐纸片扩散法（K-B）,以及随机扩增多态DNA（RAPD）技术对同期住院患者临床标本分离的30株MRSA进行同源性分析.结果 从产生的RAPD图谱分析可将30株MRSA分为5个型别,其中I型为22株占73.3%（22/30）.采用青霉素、头孢西丁、红霉素、环丙沙星、四环素、庆大霉素、复方新诺明、氯霉素、强力霉素、阿米卡星、左氧氟沙星、阿齐霉素、米诺环素、莫西沙星、亚胺培南、加替沙星、哌拉西林/他唑巴坦、美罗培南等18种抗生素,可将30株MRSA进行同源性分析.结论 RAPD技术及纸片扩散法（K-B）可以很好地用于细菌的同源性分析.

Objective To explore the homogeneity of methicillin resistant Staphylococcus （MRSA） in randomly amplified polymorphic DNA （RAPD） and dise agar diffusion （K-B method） ,and evaluate the feasibility of phenotypic method of K- B. Methods 30 collected clinical MRSA strains from synchronization patients were analysed using random primer ERIC2 with RAPD. The results were compared with K-B method. Results On the basis of bands,difference of clinical strains were analysed. 30 strians of MRSA were divided into 5 patterns. Among them type I taken 73.3% （22/30）. K-B was performed to analysed the homogeneity of MRSA by 18 kinds of antibiotics. Conclusion RAPD and K-B method is easy and reliable,they can apply to track pathogens of the hospital and study homogeneity of the bacteria.