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脱氧胆酸处理对大鼠肌肉卫星细胞的影响 被引量：1

The Effect of Deoxycholic Acid on Rat's Satellite Cells' Culture
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摘要 肌肉卫星细胞是组织特异性干细胞,负责骨骼肌的生长和再生。尽管卫星细胞的发现已有50多年的历史,但肌肉卫星细胞的维持培养仍是一个难以解决的问题。本研究使用脱氧胆酸(deoxycholic acid,DCA)处理肌肉卫星细胞,结果发现,50μmol/L脱氧胆酸能显著促进肌肉卫星细胞的增殖。Western blotting检测发现50μmol/L DCA能促进细胞内β-catenin表达水平的持续增加。处理6 d后的细胞仍然保持着肌肉卫星细胞的成肌能力。 Satellite cells are regarded as a population of muscle-specific committed progenitors that are responsible for the postnatal maintenance,growth,repair and regeneration of skeletal muscles.Although satellite cells nave been first isolated 50 years ago,the characteristic that is maintained by culture still cann＇t resolve.In this study,50 μmol/L deoxycholic acid（DCA）could promote the proliferation of rats＇ satellite cells.The expression of β-catenin increased gradually by Western blotting.The cells treated with DCA still could fusion.
作者 焦泽华 段彪 白春玲 魏著英 李光鹏
机构地区 内蒙古大学哺乳动物生殖生物学及生物技术教育部重点实验室
出处 《中国畜牧兽医》 CAS 北大核心 2010年第12期39-42,共4页 China Animal Husbandry & Veterinary Medicine
基金 国家"863"计划资助项目(2007AA100505 2008AA10Z159)
关键词 脱氧胆酸(DCA) 大鼠 卫星细胞 deoxycholic acid（DCA） rat satellite cells
分类号 S852.16 [农业科学—基础兽医学]
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参考文献15

  • 1Armstrong D D,Wong V L,Esser K A.Expression of β-catenin is necessary for physiological growth of adult skeletal muscle.Am J Physiol Cell Physiol,2006,291:C185 ～ C188.
  • 2Conboy I M,Rando T A.The regulation of Notch signaling controls satellite cell activation and cell fate determination in postnatal myogenesis.Dev Cell,2002,3:397 ～ 409.
  • 3Gavard J,Marthiens V,Monnet C,et al.Ncadherin activation substitutes for the cell contact control in cell cycle arrest and myogenic differentiation:involvement of p120 and β-catenin.J Biol Chem,2004,279:36795 ～ 36802.
  • 4Goichberg P,Shtutman M,Ben-Ze' ev A,et al.Recruitment of β-catenin to cadherin-mediated intercellular adhesions is involved in myogenic induction.J Cell Sci,2001,114:1309～ 1319.
  • 5Hirabayashi Y,hoh Y,Tabata H,et al.The Wnt/β-catenin pathway directs neuronal differentiation of cortical neural precursor cells.Development,2004,131:2791～ 2801.
  • 6Kuang S,Kuroda K,Le Grand F,et al.A symmetric self-renewal and commitment of satellite stem cells in muscle.Cell,2007,129:999～1010.
  • 7Munsterberg A E,Kitajewski J,Bumcrot D A,et al.Combinatorial signaling by Sonic hedgehog and Wnt family members induces myogenic bHLH gene expression in the somite.Genes Dev,1995,9:2911～2922.
  • 8Ozawa M,Baribault H,Kemler R.The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species.EMBO J,1989,8:1711～1717.
  • 9Petropoulos H,Skerjanc I S.β-catenin is essential and sufficient for skeletal myogenesis in P19 cells.J Biol Chem,2002,277:15393～15399.
  • 10Polesskaya A,Seale P,Rudnicki M A.Wnt signaling induces the myogenic specification of resident CD45+ adult stem cells during muscle regeneration.Cell,2003,113:841 ～ 852.

同被引文献15

  • 1Collins C A, Olsen I, Zammit P S, et al. Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche[J].Cell, 2005, 122(2): 289-301.
  • 2Fukada S, Uezumi A, Ikemoto M, et al.Molecular signature of quiescent satellite cells in adult skeletal muscle[J].Stem Cells, 2007, 25(10): 2448-2459.
  • 3Gizak A, Wrobel E, Moraczewski J, et al. Changes in subcellular localization of fructose 1,6-bisphosphatase during differentiation of isolated muscle satellite cells[J].FEBS Lett, 2006, 580(17): 4042-4046.
  • 4Montarras D, Morgan J, Collins C, et al. Direct isolation of satellite cells for skeletal muscle regeneration[J].Science, 2005, 309(5743): 2064-2067.
  • 5Pallafacchina G, Francois S, Regnault B, et al. An adult tissue specific stem cell in its niche: A gene profiling analysis of in vivo quiescent and activated muscle satellite cells[J].Stem Cell Res, 2010, 4(2): 77-91.
  • 6Qu Z, Balkir L, van Deutekom J C, et al. Development of approaches to improve cell survival in myoblast transfer therapy[J].The Journal of Cell Biology, 1998, 142(5): 1257-1267.
  • 7Sabourin L A, Rudnicki M A. The molecular regulation of myogenesis[J].Clin Genet, 2000, 57(1): 16-25.
  • 8Sacco A, Doyonnas R, Kraft P, et al. Self-renewal and expansion of single transplanted muscle stem cells[J].Nature, 2008, 456(7221): 502-506.
  • 9Seale P, Sabourin L A, Girgis-Gabardo A, et al. Pax7 is required for the specification of myogenic satellite cells[J].Cell, 2000, 102(6): 777-786.
  • 10Starkey J D, Yamamoto M, Yamamoto S, et al. Skeletal muscle satellite cells are committed to myogenesis and do not spontaneously adopt nonmyogenic fates[J].Journal Histochem Cytochem, 2011, 59(1): 33-46.

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中国畜牧兽医
2010年 第12期

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