猪流感病毒山东分离株H1N1亚型HA基因的克隆与序列分析

HA Gene Cloning and Sequence Analysis of Subtype H1N1 Swine Influenza Virus Isolated from Shandong

采用RT-PCR技术对分离的H1N1亚型猪流感病毒的HA基因进行了扩增,将获得的PCR产物与pMD18-T载体连接,进行序列测定。同源性分析结果表明,分离毒株与其他H1N1亚型猪流感病毒的HA基因核苷酸同源性为70.7%～90.8%,与A/swine/Zhejiang/1/2007的同源性最高,与其他毒株的同源性相对较低。系统进化树分析结果表明,山东分离株的HA基因与欧洲谱系猪流感病毒进化关系最近,证明该分离株可能来源于北美谱系和欧亚谱系猪流感病毒的重组。

HA gene of H1N1 subtype swine influenza virus was amplified by RT-PCR,the PCR products were obtained with the pMD18-T vector,and sequenced.Homology analysis showed that HA gene nucleotide homology of the isolated strain and other H1N1 subtype swine influenza virus were 70.7% to 90.8%,the highest homology was that with the A/swine/Zhejiang/1/2007,and homology of other strains were relatively low.Phylogenetic tree analysis showed that the evolutionary relationships of HA gene isolated in Shandong and that of European lineage of swine influenza virus were closet,proved that the isolated strain may be derived from the North American and Eurasian lineages of swine influenza virus lineage restructuring.