高压氧预处理对SPS暴露大鼠学习记忆能力以及海马神经元凋亡的影响

Effects of Hyperbaric Oxygen Preconditioning on Learning and Memory and Changes of Apoptosis in Hippocampus in SPS Rats

目的:研究高压氧(HBO)预处理对SPS暴露大学学习记忆能力及其大脑海马神经元细胞凋亡的影响。方法:48只雄性Sprague-Dawley大鼠(体重220-260 g)随机分为4组(n=12):对照(Sham)组,高压氧(HBO)组,SPS组以及高压氧+SPS组。高压氧组每天1小时高压氧预处理(2.5个大气压,100%O2)连续5天;SPS组采用单次延长应激模型;高压氧+SPS组每天1小时高压氧预处理连续5天于最后一次预处理后24小时,制作SPS模型。4组大鼠于SPS暴露后72小时进行TUNEL染色,第15天经行水迷宫测试。结果:水迷宫实验中大鼠逃避潜伏期及游泳路径四组之间有明显统计差异[F0.01(3,28)=4.88>4.57,P<0.01;F0.01(3,28)=5.31>4.57,P<0.01]。SPS组明显长于Sham组(P<0.01),而高压氧预处理能够逆转这种效应(P<0.01)。游泳速度四组之间无明显统计差异[F0.05(3,28)=2.23<2.95,P>0.05]。SPS暴露后海马神经元细胞数量和密度明显减少,给予高压氧预处理后,神经元形态明显好转,但仍不及对照组。结论:高压氧预处理可以减少海马神经元细胞凋亡从而改善SPS暴露后大鼠认知功能障碍。

Objective：To investigate the effect of Hyperbaric oxygen（HBO） preconditioning on learning and memory damage in-duced by SPS exposure in rats.Methods：Forty-eight male Sprague-Dawley rats were randomly divided into four groups with 12 in each group：Sham group,SPS group,HBO group and HBO＋SPS group.HBO-PC rats were conducted by exposing to 100% oxygen at 2.5 atm absolute for 1 h each day for 5 consecutive days.SPS was performed 24 h after the last HBO-PC conditioning event.TUNEL staining was performed at 72 h after SPS.Morris water maze was used to observe the navigation and probe capabilities of rats.Results：Compared with the Sham group,the escape latency increased significantly in the SPS and HBO＋SPS groups（P0.05） while which had no difference in HBO group.HBO＋SPS group had significantly shorter escape latency than that of the ＋SPS group（P0.05）.Spatial search capability of the rats in HBO＋SPS group was better than that in SPS group.Conclusion：HBO preconditioning may have a protective effect on the impairment of learning and memory and reduce apoptosis in hippocampus caused by SPS exposure in rats.