NF-κ B和HIF-1在宫颈上皮内瘤变和鳞癌中表达的意义

Clinical pathological Study on NF-κ B and HIF-1 Expression in cervical intraepithelial neoplasia and squamous cell carcinoma of the cervix

目的:初步探索NF-κB和HIF-1在宫颈上皮内瘤变和宫颈鳞癌中的表达及临床病理学意义。方法:以宫颈上皮内瘤变和宫颈鳞癌病例为研究对象,应用免疫组织化学方法检测NF-κB和HIF-1的表达情况并分析其表达在宫颈癌变中的意义。结果:NF-κB在正常宫颈上皮、CINⅠ、CINⅡ、CINⅢ、鳞癌的阳性表达率分别为16%,36%,44%,68%,72%。总体比较存在显著性差异x2=21.636,p<0.01。组间两两比较显示正常上皮与CINⅢ和鳞癌有显著性差异。HIF-1α在正常宫颈上皮、CINⅠ、CINⅡ、CINⅢ、鳞癌的阳性表达率分别为12%,20%,40%,68%,76%。总体比较存在显著性差异x2=32.733,p<0.01。组间两两比较显示正常上皮与CINⅢ(x2=16.333 p<0.001)和鳞癌(x2=20.779 p<0.001)有显著性差异。结论:NF-κB和HIF-1与宫颈鳞癌的发生有关,可能作为早期诊断的标志物。

Objective：Clinical pathological significance of NF-κB and HIF-1 expression was investigated.Methods：NF-κB and HIF-1 expression were assayed by immunohistochemical method and its significance was evaluated in cervical intraepithelial neo-plasia and squamous cell carcinoma of the cervix.Results：NF-κB positive expression rate in normal cervical epithelium,CINⅠ,CINⅡ,CINⅢ,squamous cell carcinoma were respectively 16%,36%,44%,68%,72%,which significant difference exits（x2=21.636,p0.01）,and there is significant difference between normal cervical epithelium and CINⅢ,squamous cell carcinoma.HIF-1αpositive ex-pression rate in normal cervical epithelium,CINⅠ,CINⅡ,CINⅢ,squamous cell carcinoma were respectively 12%,20%,40%,68%,76%,which significant difference exits（x2=32.733,p0.01）,and there is significant difference between normal cervical epithelium and CINⅢ,squamous cell carcinoma.Conclusions：NF-κB and HIF-1 were related to cervical squamous cell carcinoma and may be biomarkers for early diagnosis.