人参皂甙对缺氧大鼠海马DG区神经细胞DNA损伤保护作用的研究

Protective Function of Panaxosideon on DNA Damage of Hippocampus Neurocyte in Dentate Gyrus of Hypoxia Exposed Rats

目的:用单细胞凝胶电泳技术(SCGE)研究急慢性缺氧大鼠海马DG区神经细胞细胞DNA损伤和人参皂甙对缺氧大鼠海马细胞DNA的保护作用。方法:健康成年SD大鼠随机分为急、慢性缺氧正常对照组、急性缺氧组和慢性缺氧组(分别在模拟海拔5000米高原环境连续缺氧暴露0d、3d和30d)、急性缺氧人参皂甙干预组、慢性缺氧人参皂甙干预组,应用SCGE检测海马DG区神经细胞DNA损伤。结果:随着缺氧时间的增加,海马DG区神经细胞DNA的损伤程度加重,尾长、尾部DNA百分含量和尾距显著增加(P<0.05)。人参皂甙能使缺氧损伤的海马DG区神经细胞的尾长、尾部DNA百分含量和尾距均较缺氧组减少(P<0.05)。结论:人参皂甙能有效地减轻缺氧引起的海马组织细胞DNA的断裂损伤。

Objective：To observe the protective function of panaxosideon on DNA damage of hippocampus neurocyte in the den-tate gyrus（DG） of hypoxia exposed rats by the single cell gel electrophoresis（SCGE）.Methods：Adult SD rats were randomly divided into six groups（acute control,chronic control,acute hypoxia,chronic hypoxia,acute hypoxia with panaxoside,chronic hypoxia with panaxo-side groups）.The animals were placed into a hypobaric chamber simulated 5000 m high altitude for 0d,3d and 30 d,respectively.SCGE was used to detect the DNA damage.Results：The longer the time of hypoxia exposed,the more the DNA damaged of hippocampus neu-rocyte in the dentate gyrus（DG）,the more the tail length,the tail DNA content and the tail moment（P0.05）.panaxosideon could signifi-cantly decrease the tail length,the tail DNA content and the tail moment of damaged cells（P0.05）.Conclusions：Panaxosideon could could reduce effectively DNA damage of hippocampus neurocyte in the dentate gyrus（DG） of hypoxia exposed rats.