N-乙酰半胱氨酸对大鼠肝脏缺血再灌注损伤NF-κ B和ICAM-1表达的影响

Effect of N-acetylcysteine on Expression of NF-κ B and ICAM-1 in Rats with Hepatic Ischemia/Reperfusion Injury

目的:探讨大鼠肝脏缺血再灌注损伤NF-κB和ICAM-1表达情况及NAC的保护作用机制。方法:45只雄性SD大鼠随机分成三组:假手术组(Sham组,n=5);缺血再灌注损伤组(I/R组,n=20)缺血60min后分别再灌注1、3、6、12h;N-乙酰半胱氨酸组(NAC组,n=20):先自阴茎背静脉给大鼠注射溶于生理盐水的NAC,20min后再按I/R组处理。在各规定的再灌注时间点,分别采用western-blot和免疫组化方法测定肝组织中NF-κB和ICAM-1的表达。结果:I/R组和NAC组再灌注1、3、6、12h后,NF-κB的表达均明显高于Sham组(p<0.01),于再灌注3h达到高峰;ICAM-1的表达均明显高于Sham组(p<0.01),于再灌注6h达到高峰。NAC组再灌注1、3、6h与I/R组相同时间点比较:NF-κB和ICAM-1的表达均低于I/R组(p<0.05)。NAC组再灌注12h与I/R组相同时间点比较:NF-κB和ICAM-1的表达虽然在数值上有所减少,但统计学上无差异(p>0.05)。结论:大鼠肝脏缺血再灌注后NF-κB和ICAM-1表达增加,NAC可抑制NF-κB激活,减少ICAM-1表达减轻大鼠肝脏缺血再灌注损伤。

Objective：To investigate the expression of NF-κB and ICAM-1mechanisms in the Hepatic Ischemia/Reperfusion injury of rats,and the protective mechanisms of NAC.Methods：Forty-five male SD rats were randomly divided into three groups：sham operation group（Sham group n=5）;Ischemia-Reperfusion group（I/R group n=20）：Anaesthetized rats were subjected to 60min of sus-tained occlusion of the blood for left and middle liver followed by 1 hours,3 hours,6 hours and 12 hours of reperfusion respectively;（3）NAC group（N group n=20）：NAC dissolved in the saline was injected from dorsal vein of penis,then rats were dealled as the I/R group 20min later.At the end of reperfusion,liver tissues samples were obtained for detection the experssion of NF-κB and ICAM-1 by meth-ods of western-blot and immunohistochemistry.Results：After reperfusion1h,3h,6h and 12h later,the expression of NF-κB in I/R and NAC groups were higher than Sham group（P0.01）,and reached the peak after reperfusion 3h;The expression of ICAM-1 were all high-er than Sham group（P0.01）,and reached the peak at reperfusion 6h.At 1h,3h,6h after reperfusion of NAC group,the expression of NF-κB and ICAM-1 were lower than the same time of I/R group（P0.05）.At 12h after reperfusion,the the expression of NF-κB and ICAM-1 seem lower than the the same time of I/R group,but there was no difference between the two groups（p0.05）.Conclusion：The expression of NF-κB and ICAM-1 increased obviously in Hepatic Ischemia/Reperfusion of rat,NAC can attenuate Hepatic Is-chem/Reperfusion injury by inhibiting the activation and expression of NF-κB and reduce the expression of ICAM-1.