精胺对放射损伤防护作用的实验研究

Experiment Study of Protective Effects of Spermine on Radiaton Injury

目的探讨精胺对放射损伤的保护机制及可能的治疗效果。方法昆明种雄性小鼠随机分为对照组（Ａ组），单放组（Ｂ组），放射前注入精胺组（Ｃ组），放射后注入精胺组（Ｄ组）；６Ｇｙ一次性全身^（６０）Ｃｏｒ射线照射后，在１５ｄ内观察各组小鼠体重、存活率、以及外周血和骨髓细胞数量的变化；用ＥＬＩＳＡ和放免的方法分别检测血清ＴＧＦ－β_１和ＴＮＦ－α的含量；用免疫组化法检测骨髓组织ＴＮＦ－α和ＴＧＦ－β_１的表达。结果①Ｃ、Ｄ两组小鼠的体重明显高于Ｂ组，存活率显著提高（ Ｐ＜０．０１）；②Ｃ、Ｄ两组血清及骨髓组织中ＴＮＦ－α和ＴＧＦ－β_１水平较Ｂ组显著降低。结论精胺对放射损伤的保护作用与其抑制造血抑制因子表达有关。

Objective:To explore the protective mechanism of spermine to radiaton injuryed mice of spermine on radiation injury mice Methods Kunming strain mice, divided into A group(Control). B group(radiation injury) . C group(injected spermine before irradiation). D group(injected spermine after irradiation), were given whole body irridiation of γ-ray from^(60)C_(or)in a dose of 6Gy, then serum and bone marrow tissue. TNF-a and TGF-β_1 were assayed with ELISA. Radio - immunity and immuno - histochemic methods respectively. At same time, the weight was weighed. fatal rate and some cell count in blood or bone marrow were observed. Results: (1)C and D group mice were not only more heavy in weighr but also hiogher in survival rate than B group' s. (2)The TNF-α and TGF-β_1 level in bind and bone marrow tissue o C.D were markediy lew- er than those in B group. Conclusion:The protective effect of sermine on radiaton injury was associated with its inhibiting function expres- sion of hematopietic inhibitors.