分泌性中耳炎鼻咽部免疫球蛋白的研究

A Study of Nasopharynx Immumo globulins in Otitis Media Effusion

目的：研究分泌性中耳炎发病机制中免疫因素的作用。方法：本实验采用速率散射比浊法及酶联免疫测定法测定了正常人及分泌性中耳炎患者鼻咽部灌洗液中免疫球蛋白ＩｇＧ、ＩｇＡ、ＩｇＭ、ＩｇＥ含量。结果：鼻咽部灌洗液中ＩｇＧ、ＩｇＭ、ＩｇＥ的含量与自身外周血中的相应抗体呈正相关（ｒ_１＝ ０． ９１、ｒ_２＝ ０． ８９、ｒ_３＝ ０． ８７），而 ＩｇＡ的含量无相关性（ｒ_４＝ ０． ２５）；灌洗液中四种抗体间无相关性；分泌性中耳炎患者鼻咽部灌洗液中四种抗体的含量均高于正常对照组，而分泌性中耳炎患者年龄与病程对四种抗体含量影响不大。结论：分泌性中耳炎患者鼻咽部存在持续的免疫刺激因素。

Objective: Analysis of immunoglobulins in otitis media effusion. Methods: Concentrations of IgG. IgA.IgM. and IgE in the. nasopharynx irrigated wash liquid and serum samples for normal control and OME were detemtined by nephelometry and ELISA. Results: The concentraion of IgG.IgM and IgE in the nasopharynx irrigated wash liquid were the right relative of the sexum samples itself(r_1 = 0. 91.r_2 = 0. 89). r_3 = 0. 87)and concentration of IgA has not at the relative of the serum samples itself(r_4 = 0. 25). There were not the relative of IgG. M. IgM and IgE in the nasopharynxirrigated wash liquid. Concentrations of IgG. IgA.IgM and IgE of the nasopharyns irrigated wash liquid in OME were higher than the nomal control. Especially the concentration of IgA increased significantly. The concentrations of the four immunoglobulins were not associated with the age and course of OME. Conclusion: There was a sustained immunization in the nasopharynx of OME.