荧光定量PCR法在检测246例小儿巨细胞病毒感染中的应用

Application of fluorescent quantitative PCR in detection of cytomegalovirus infection in children

目的:探讨FQ-PCR在诊断小儿巨细胞病毒感染的临床应用价值。方法:分别应用血清抗体检测和荧光定量聚合酶链反应(FQ-PCR)的方法对患者进行血液和尿液病毒的检测,并对确诊的患者进行母乳CMV病毒的检测。结果:在怀疑为活动性CMV感染的169例患儿中,PCR法的阳性检出率为75.1%,血清IgM法为52.1%,两种方法的阳性符合率为86.4%;18例尿液CMV-DNA阳性的患者查出母亲乳汁中CMV-DNA,含量为1.7×10～4.5×103copies/mL。结论:FQ-PCR可以准确检测患儿体液及血液中的CMV含量,提示体内复制情况,对于临床的诊断和治疗有一定的指导意义。

Objective：To explore the significance of fluorescent quantitative PCR in the Diagnosis of cytomegalovirus infection in children.Methods：CMV-DNA from urine and peripheral blood leukocytes was detected by FQ-PCR and serum anti-CMV IgM was detected by ELISA,respectively.Results：All 169 suspected active HCMV-infected children were examined by flurescent quantitative PCR and ELISA to detect anti-CMV IgM in serum.The positive rates of FQ-PCR and ELISA were 75.1% and 52.1%,respectively,18 specimens of breast milk were positive by FQ-PCR compared with the urine CMV-DNA.The DNA copy number was 1.7×10～4.5×103.Conclusion：FQ-PCR can evaluate CMV viral load accurately,and be used to monitor the true state of CMV infection and antiviral therapy.