中药复方中葛根素在肉鸽体内的代谢特征研究

Metabolic Characteristics on Puerarin of Chinese Herbal Compound in Meat Pigeons in vivo

运用高效液相色谱法测定分析了中药复方(葛银方剂)中葛根素在肉鸽体内的代谢情况,以120mg/只灌胃后通过药动学参数变化来评价药的相互作用。测定血药浓度,并用3P87程序拟合药动学参数。结果发现,单味煎剂和中药复方灌服后15min血浆中即可检出葛根素,在15～4h才会出现药浓度最高峰。葛根素在肉鸽体内呈二室模型分布,单味煎剂中葛根素主要药动学参数为t1/2α=1422022h,t1/2β=7862796h,Cmax=0732025μg/L,Tpeak=1818334h;中药复方中葛根素主要药动学参数是t1/2α=2128758h,t1/2β=5108140h,Cmax=1037176μg/L,Tpeak=1383717h。结果表明,中药复方中葛根素在肉鸽体内的代谢具有速度快、吸收强的特点。

High performance liquid chromatography（HPLC） was used to study the metabolic characteristics of puerasin of Geyin compound preparation in meat pigeons,which were given 120 mg puerasin per kg of pigeon weight by stomach catheter,and then evaluated the interaction effects with pharmacokinetic parameters. Puerarin concentration was detected and its pharmacokinetic parameters were calculated and analyzed by 3P87 program. The puerarin could be determined after 15 min of given medicine and the maximum concentration appeared around 1.5 to 4 h. The data of drug-time of puerarin were accord with two-compartment model. The main pharmacokinetic parameters of liquid preparation were t1/2α=1.422022 h,t1/2β=7.862796 h,Cmax=0.732025 μg/L,Tpeak=1.818334 h;on the other hand,the main pharmacokinetic parameters of Chinese herbal compound were t1/2α=2.128758 h,t1/2β=5.108140 h,Cmax=1.037176 μg/L,Tpeak=1.383717 h. The results showed that puerarin of Geyin compound preparation could be absorbed quickly and had great absorption effects.