电离辐射诱发的Jurkat T淋巴细胞凋亡及其机制

Apotosis and its mechanism of Jurkat T lymphocytes induced by ionizing radiation

用原位末端标记法（ＴＵＮＥＬ） 、麦格—姬姆萨（ ＭＧＧ） 染色法和免疫组化技术， 观察了经５、１０ 、２０和３０Ｇｙ 不同剂量的γ射线照射后４ ｈ～３６ ｈ， 人Ｊｕｒｋａｔ Ｔ淋巴细胞白血病细胞（简称Ｊｕｒｋａｔ 细胞） 的凋亡及Ｂａｘ和Ｂｃｌ２ 蛋白的表达。结果表明， 不同剂量γ射线照射后， Ｔ淋巴细胞数的减少和凋亡率均显示出较好的量效和时效关系， 证实细胞凋亡是照射后Ｔ淋巴细胞数减少的主要原因之一； Ｂａｘ 和Ｂｃｌ２ 蛋白在上述电离辐射诱发的Ｊｕｒｋａｔ 细胞凋亡的调控中起着重要的作用。

Using TUNEL assay,May grunwald Giemsa(MGG) staining and immunohistochemical technique,we studied the apoptosis and its mechanism of Jurkat T lymphocyte leukemia cells irradiated by 5,10,20 and 30 Gy γ rays The results showed that, therewere good irradiation dose dependent and time dependent effect relationships upon the T cell subpopulation reduction and apoptotic rate of T cells, showing that apoptosis might be one of major causes of T lymphocyte decrease after radiation;Bax and Bc1 2 proteins played important roles in the regulation of Jurkat T cell apoptosis induced by ionizing radiation