番石榴4个品种间的遗传多样性RAPD分析

RAPD Analysis of Genetic Diversity of 4 Varieties of Psidium guajava L.

应用RAPD技术对番石榴4个品种的遗传多样性和亲缘关系进行分析,从50个随机引物中筛选出7个多态性明显、反应稳定的引物,共扩增出49条谱带,且全部为多态性谱带,对扩增出来的49条带进行统计分析、计算遗传距离,并PAUP4.0b10软件包选择UPGMA法对所得到数据矩阵进行聚类分析.结果表明4个品种之间的遗传距离为0.154 74-0.451 61,存在明显遗传差异,并且它们可以被分为两大类.

The random amplified polymorphic DNA（RAPD） technique was used to analyze the genetic diversity and phylogenetic relationship of 4 varieties of Psidium guajava.7 primers which had good repeat and clear bands were screened from 50 arbitrary 10-mer primers.The total 49 bands were obtained and these bands all displayed polymorphism.49 RAPD bands were recorded and the obtained characters data were used to calculate genetic distances.The cluster analysis was conducted by using PAUP＊4.0b10 software with the unweighted pair-group method of arithmetic averages（UPGMA）.The results showed there were genetic diversities with genetic distances from 0.154 74 to 0.451 61 among 4 varieties of Psidium guajava and they can be classified into two groups.