板党硒多糖对大鼠肢体缺血/再灌注后心肌损伤的影响

Effects of Se-Polysaccharides in Codonopsis Pilosula from Banqiao on Myocardium Injury in Rat Suffering from Limb Ischemia Reperfusion

目的研究板党硒多糖(Se-Polysaccharides in Codonopsis Pilosula from Banqiao)对肢体缺血/再灌注(1imbischemia reperfusion,LIR)后心肌损伤的影响。方法 SD大鼠随机分为三组:对照组、模型组和板党硒多糖治疗组。对照组和模型组用生理盐水灌胃处理,板党硒多糖治疗组用板党硒多糖灌胃处理,4周后,模型组和板党硒多糖治疗组造成LIR损伤,观察再灌注4h后左心室内压力峰值(LVSP)、左心室舒张末期压(LVEDP)和左心室内压最大变化速率(±dp/dtmax),血中乳酸脱氢酶(LDH)、α-羟丁酸脱氢酶(α-HBDH)、肌酸激酶(CK)含量的变化,血中超氧化物歧化酶(SOD)和谷光甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量的变化。结果 4 h后,模型组与对照组比较:LVSP和±dp/dtmax降低、LVEDP增高(P<0.01、P<0.05);SOD和GSH-Px活性明显降低、MDA含量明显增加(P<0.01);LDH、α-HBDH和CK均明显增高(P<0.01)。板党硒多糖治疗组与模型组比较:LVSP和±dp/dtmax增高(P<0.01、P<0.05)、LVEDP变化不明显;SOD和GSH-Px活性增高、MDA含量减少(P<0.01);LDH、α-HBDH和CK均降低(P<0.01、P<0.05)。结论板党硒多糖对LIR后心肌损伤有明显的保护作用。

Objective To investigate the effects of Se-Polysaccharides in Codonopsis Pilosula from Banqiao on myocardium injury in rat which suffered from limb ischemia reperfusion（LIR）.Methods SD rats were divided into three groups： control group,model group and treatment group.The control group and model group were given NaCl by gavages and the treatment group by Se-Polysaccharides in Codonopsis Pilosula from Banqiao respectively for 4 weeks,and then LIR models were established.Observations were made on left ventricular systolic pressure（LVPS）,left ventricular end-diastolic pressure and ±dp/dtmax after reperfusion 4h as well as the activity of SOD,GSH-Px,LDH,α-HBDH and CK,the amount of MDA after reperfusion.Results Compared with control group,LVSP and ±dp/dtmax went down in model group,LVEDP（P0.01,P0.05） went up;the activity of SOD,GSH-Px,LDH,α-HBDH and CK were greatly lowered,the amount of MDA went up（P0.01）.While in comparison with model group,LVSP and ±dp/dtmax went up significantly in treatment group（P0.01,P0.05）;the activity of SOD,GSH-Px,LDH,α-HBDH and CK went up,but the amount of MDA went down（P0.01,P0.05）.Conclusion Se-Polysaccharides in Codonopsis Pilosula from Banqiao can greatly protect the myocardium from limb ischemia reperfusion.