基于聚硫堇的一次性O型口蹄疫抗原酶免疫传感器的研制

Fabrication of Enzyme Immunosensor for Detection of Type-O FMDV-Ag Based on Poly(Thionine) Modified Screen-Printed Carbon Electrode

将O型口蹄疫酶标抗体掺杂于有机-无机溶胶凝胶中,并修饰于聚硫菫的丝网印刷碳电极表面,从而制备一次性口蹄疫病毒抗原(FMDV-Ag)酶免疫传感器。试验中采用直接免疫分析法检测口蹄疫病毒抗原。根据免疫反应前后还原峰电流下降的百分率K值的大小实现对抗原的检测。在优化条件下,免疫传感器检测抗原的线性范围为77～388ng/mL,最低检出限为32ng/mL。免疫电极具有较好的特异性、重现性(RSD=5.6%)、稳定性(10d后电流响应为初始值的89.5%)和准确性(与AGP符合率为95%)。因此,该免疫传感器有望用于O型口蹄疫抗原的快速检测。

A disposable electrochemical immunosensor for detection of FMDV based on the poly（thionine） modified screen-printed electrode coated with Chitosan-silica sol-gel doped with HRP-anti-FMDV has been developed in the experiment.Then the immunosensor was characterized by cyclic voltammetry（CV）.According to the decrease percentage of response current,type-O FMDV-Ag could be detected.Under the optimum conditions,type-O FMDV-Ag can be rapidly detected by sigmoidal curve method and form a linear calibration between 77 to 388 ng/mL with an associated detection limit of 32 ng/mL（S/N=3）.The immunosensor had acceptable specificity,reproducibility（RSD=5.6%）,stability（the amperometric response was 89.5% of the initial response after 10 d） and accuracy（95% of the results obtained by the immunosensor were in agreement with those obtained by AGP）.Therefore,the immunosensor could be hopefully used to rapidly detect type-O FMDV-Ag.