摘要
目的观察Rho激酶(ROCKS)特异性抑制剂Y-27632对体外培养的U87胶质瘤细胞迁移能力的影响。方法体外培养U87胶质瘤细胞,采用MTT实验、Transwell体外细胞迁移实验,检测Rho激酶抑制剂Y-27632对人U87胶质瘤细胞生长和迁移能力的影响。Rho激酶检测试剂盒检测Y-27632对ROCKS活性的抑制率。结果 Y-27632对U87细胞生长活性无明显影响;细胞迁移能力明显下降,与对照组相比差异性显著,统计学处理P<0.05。随着Y-27632浓度的增加,细胞中ROCKS活性逐渐抑制,呈浓度依赖性。结论 Rho激酶抑制剂Y-27632能明显抑制U87胶质瘤细胞的体外迁移能力。
Objective To explore the effects of Y-27632,inhibitor of Rho-kinase on proliferation and migration of U87 glioblastoma cells in vitro. Methods U87 cells were treated with Y-27632 of different concentrations in vitro,cell proliferation inhibition was detected by MTT colorimetric assay;cell migration inhibition was detected by cell motility assay.Inhibition of Rho-kinase was detected by Rho-kinase Assay Kit. Results The activity of U87 glioblastoma cell was not disturbed.Cell migration was suppressed strongly.Y-27632 could decrease the activity of Rho-kinase with dose-dependence. Conclusion Y-27632,inhibitor of Rho-kinase could significantly inhibit the migration of U87 glioblastoma cell in vitro.
出处
《河南科技大学学报（医学版）》
2010年第4期247-249,252,共4页
Journal of Henan University of Science & Technology:Medical Science
