CD4^+ Treg细胞在不稳定型心绞痛中的变化及其意义

Alterations and Significance of CD4^+ Treg Cell in Unstable Angina(UA)

目的研究Treg细胞在不稳定型心绞痛中(UA)的表达及其意义,并探讨Treg细胞在不稳定型心绞痛患者与健康人之间的差异。方法流式细胞仪检测CD4+CD25+T细胞和CD4+FOXP3+T细胞分别占CD4+T细胞的比例,采用直接免疫荧光法,以winmdi29软件系统分析。结果 CD4+FOXP3+T细胞占CD4+T细胞的比例:UA组低于正常对照组(t=8.43,P<0.01)差异有统计学意义;CD4+CD25+T细胞占CD4+T细胞的比例:UA组明显低于正常对照组(t=14.67,P<0.01),差异有统计学意义。结论 Treg细胞数量在UA组中明显减少,提示不稳定型心绞痛患者的免疫系统存在异常。

Objective To explore the alterations and significance of CD4＋T lymphocyte（Treg cells） in unstable angina as well as to discuss the distinction of Treg cells between UA patients′ and healthy people′s.Methods The percentages of CD4＋CD25＋T cells and of CD4＋FOXP3＋T cells in CD4＋T cells were examined by direct immunofluorescence technique and detected by flow cytometry and analysed by winmdi29 software system.Results The proportions of CD4＋FOXP3＋T cells in CD4＋T cells were lower in UA patients than in normal control（t=8.43,P0.01）.The percentages of CD4＋CD25＋T cells in CD4＋T cells were obviously lower in UA patients than in normal control（t=14.67,P0.01）.Both of them had a statistically significance.Conclusions The quantity of CD4＋Treg cells was obviously smaller in UA patients than in normal control and it could be inferred that the immune system of unstable angina patient was abnormal.