胃癌患者外周血癌胚抗原mRNA的检测及临床意义

Detection of CEA mRNA in peripheral blood of patients with gastric cancer and its clinical significance

目的 探讨胃癌患者外周血中癌胚抗原（carcinoembryonic antigen,CEA）mRNA的表达及临床意义.方法 采用巢式RT-PCR方法检测50例胃癌术前患者、20例健康志愿者外周血以及10例新鲜胃癌组织中CEA mRNA的表达,并同期测定胃癌患者及健康志愿者血清CEA水平.结果 胃癌患者外周血中CEA mRNA表达阳性率为48.00%,明显高于外周血中的CEA阳性率20%,差异有统计学意义（P＜0.05）;胃癌患者外周血中CEA mRNA阳性率与组织学类型、临床分期具有相关性（P＜0.05）,而与患者性别、年龄及有无淋巴结转移无明显相关性（P＞0.05）,20例健康志愿者无阳性表达,10例胃癌组织均为阳性表达.结论 检测外周血中的CEA mRNA表达可能是检测胃癌微转移一种较理想的方法,对判断患者预后及进一步治疗有积极意义.

Objective To investigate the expression of CEA mRNA in peripheral blood of patients with gastric cancer and its clinical significance. Methods The expression of CEA mRNA was detected in gastric cancer tissues （n = 10） and in the peripheral blood of patients（n = 50） with gastric cancer before the operation and 20 healthy donors by nested - reverse transcription polymerase chain reaction. Also the level of serum CEA was evaluated in patients with gastric cancer and 20 healthy donors. Results The positive rate of CEA mRNA in the peripheral blood of preoperative patients with gastric cancer was 48.0% ,which was significantly higher than the positive rate of CEA in the peripheral blood in the healthy control （20%） （P 〈 0. 05 ）. The positive rate of CEA mRNA was correlated with the pathologic differentiation and clinical states（P 〈 0.05 ） but not with the gender, age or lymph node metastasis （P 〉 0.05 ）. The expression of CEA mRNA was positive in all the gastric cancer tissues, but negative in the peripheral blood of healthy subjects. Conclusion The micrometastasis of gastric cancer may be detected through the evaluation of expression of CEA mRNA in peripheral blood by nested - reverse transcription. Detection for CEA mRNA may help evaluate the prognosis and suggest advanced therapy.