摘要
目的 检测K-ras基因12位点突变和p21蛋白表达在直肠癌中的作用,并探讨K-ras基因12位点突变、p21蛋白表达与临床、病理参数之间的关系.方法 收集52例直肠癌手术标本,应用常规方法提取直肠癌组织和相应癌周正常组织的DNA、聚合酶链反应(PCR)扩增、单链构象多态性(SSCP)电泳,检测K-ras基因12位点的突变情况,用免疫组织化学方法检测p21蛋白的表达.结果 直肠癌发生K-ras基因12位点突变为44.2%,相应癌周正常组织中未检出K-ras基因12位点突变,差异有统计学意义(P<0.01).p21蛋白表达率为63.46%,明显高于相应癌周正常组织的表达率,差异有统计学意义(P<0.01).K-ras基因突变与性别、年龄、肿瘤大小、肿瘤浸润深度、组织学类型、淋巴结转移、远处转移无关.p21蛋白高表达与淋巴结转移密切相关(P<0.01),与远处转移有关(P<0.05),与患者性别、年龄、肿瘤大小、肿瘤浸润深度、组织学类型无关(P>0.05).结论 K-ras基因12位点的突变是直肠癌发生的重要事件,可作为一种诊断标志物.p21蛋白表达与直肠癌发生密切相关,可作为一种免疫标志物,并可用于判断直肠癌的预后.
Objective To examine the mutation of the K- ras 12th codon and p21 expression in the pathogenesis of rectal cancer,and the relations among the K -ras codon 12 mutation, the p21 expression and the clinicopathological parameters. Methods A total of 52 samples of rectal cancer were detected for the mutation of the K -ras 12th codon and the p21 expression by PCR, SSCP and immunochemistry respectively. The relations among the K - ras codon 12 mutation, the p21 expression and the clinicopathological parameters were analyzed. Results The rate of the K - ras codon 12 mutation was 44.2 % , but no such mutation was found in the adjacent normal tissue ( P 〈 0.01 ). The rate of p21 expression was 63.46%, which was much higher than that in the normal tissue ( 3.85 % ) ( P 〈 0. 01 ). The K - ras codon 12 mutation was not related to the gender, age, tumor size, infiltration, histological type, lymph node metastasis, distant metastasis (P 〉 0.05 ). The expression of p21 was related to lymph node metastasis ( P 〈 0.01 ) and distant metastasis ( P 〈 0.05 ) but not to gender,age, tumor size, infiltration and histological type ( P 〉 0.05 ). Conclusion The mutation of the K - ras 12th codon may be the early event in the development of rectal cancer, and can be used as a diagnostic marker. As the p21 expression correlates closely to the morbidity of rectal cancer,it may be used as an immunohistochemical marker for estimating the prognosis of rectal cancer.
出处
《临床外科杂志》
2010年第12期826-828,共3页
Journal of Clinical Surgery
基金
基金项目:湖北省自然科学基金资助项目(No:2008CDB129)