母乳中多氯联苯的污染水平与特征研究

Level and congener profile of polychlorinated biphenyls in breast milk from Shaoxing,Zhejiang

目的:研究母乳中多氯联苯的污染水平和特征。方法:母乳经冷冻干燥后,加入同位素内标,经索氏抽提萃取,再经凝胶色谱、酸碱硅胶柱和氧化铝柱净化,用高分辨气相色谱-高分辨磁质谱联用仪测定,同位素内标法定量分析母乳中的多氯联苯残留。结果:母乳中18种PCB的总浓度均值为23881±9718 pg/g lipid(13643～45205 pg/g lipid),毒性当量为3.56±1.06 pg/g lipid(2.92～6.31 pg/g lipid)。PCB-138(37.58%),PCB-153(19.07%),PCB-118(11.79%),PCB-180(7.70%),PCB-105(7.00%)依次是检出浓度最高的前五种单体。结论:绍兴母乳中多氯联苯的含量与国内其它报道的数据比较接近,但显著低于发达国家,推测膳食摄入是人体暴露PCBs的一个主要途径。

Objective：To investigate level and congener profile of polychlorinated biphenyls in breast milk from Shaoxing,Zhejiang province.Methods： Breast milk was added 13C labeled internal standard after freeze-drying.Further cleanup procedure was followed by gel permeation chromatograph（GPC）,multilayer silica column and an alumina column.Sample was analyzed by HRGC-HRMS under SIM mode.Results： Mean concentration of 18 PCBs in breast milk was 23881±9718 pg/g lipid（range： 13643-45205 pg/g lipid）.The toxic equivalency（TEQ） was 3.56±1.06 pg/g lipid（range： 2.92-6.31 pg/g lipid）.PCB-153（19.07%）,PCB-118（11.79%）,PCB-180（7.70%）,and PCB-105（7.00%） were five most abundant congeners.Conclusion： PCB level found in this study is similarly to those reported elsewhere from China,but obviously lower than those from developed countries.Dietary intake is suspected as main route of human PCB exposure.