有偿供血者HIV感染分子流行病学研究

Molecular epidemiological study on HIV infection among paid blood donors

目的:了解河北省有偿献血员中HIV-1基因亚型特点和传播途径。方法:采集HIV感染者的全血样品,分离外周血单核细胞(PBMC),提取前病毒DNA,使用套式聚合酶链反应(nested-PCR),扩增HIV-1的env基因的C2-V3区并进行序列测定和亚型分析。结果:对7份HIV-1感染者的样品,扩增得到了7份HIV-1 env C2-V3基因片段,经序列测定和基因分析7份样品均为B′亚型。组内基因离散率8.83%±4.01%(n=7),基因序列与云南瑞丽株rl42(泰国B亚型)相近。V3环顶端四肽存在3种类型:GPGQ(4/7)、GPGR(2/7)以及GPGK(1/7)。结论:在河北省有偿献血员中B′亚型仍是主要的流行亚型,可能来源于云南吸毒人群。V3环顶端四肽以GPGQ为主。

Objective：To understand the characteristics,origin and transmission pattern of subtypes of HIV-1 strains infected paid blood donation in Hebei Province.Methods： We collected the whole blood from HIV-1 infected individuals and separated the peripheral blood mononuclear cell.The provirus DNA was extracted and amplified by nested-PCR,after which the HIV-1 env C2-V3 gene fragments was sequenced and its subtypes was analyzed.Results： Among 7 HIV-1 antibody positive cases,7 HIV-1 env C2-V3 gene fragments were amplified and analyzed.7 cases were confirmed to be infected with HIV-1 subtype B′ strain.The genetic distance of HIV-1 B′ intersubtype is 8.83%±4.01%（n=7）,their gene sequence were closely related to rl42 in Yunnan（Thailand B）.7 HIV-1 strains had 3 types of V3 loop tetra peptide： GPGQ（4/7）,GPGR（2/7） and GPGK（1/7）.Conclusion： HIV 1 subtype B′ was the predominant subtype circulating among paid blood donation in Hebei Province.The subtype B′ might be transmitted by drug users of Yunnan.The V3 loop tetra peptide was mostly GPGQ.