HPLC测定双份饭中的维生素A

Determination of Vitamin A in Duplicate Diets by HPLC

目的:建立高效液相色谱法测定双份饭中维生素A含量的测定方法。方法:称取10 g样品于平底烧瓶中,加入淀粉酶,混匀,保持45℃30 min。经没食子酸乙醇-氢氧化钠皂化,石油醚提取,40℃通氮气情况下旋转蒸发近干,石油醚溶解、定容,再用氮气吹干,甲醇定容。HPLC法分析,保留时间定性,标准曲线法定量。分析柱为ODS柱(250 mm×46 mm,5μm),流动相:甲醇+水(98+2),检测波长325 nm。结果:维生素A在0.25μg/ml～110μg/ml之间线性良好(r>0.9994),平均回收率为97.3%,RSD为1.2%。结论:HPLC法测定双份饭中维生素A的含量简便、快速,灵敏度高,准确性好。

Objective：An analysis that determination of Vitamin A in duplicate diets by HPLC was constructed.Methods： 10 g samples was weighed into florence flask and amylase was added.Then well mixing and keeping 30 minute at 45℃.Saponified by gallic acid ethanol-sodium hydroxide and extracted by petroleum ether.Then rotary evaporated close to dry by nitrogen-blow at 40℃.Dissolved and diluted with petroleum ether to volume.Then evaporated to dry by nitrogen-blow again and diluted with methanol to volume.Analyzed by HPLC,qualitated by retention time and quantitated by standard curve method.The analysis column of this method was ODS column（250 mm×46 mm,5 μm）,the flow phase was methanol：H2O（98∶2） and detection wavelength was 325 nm.Results： The calibration plot was found to be linear in the range of 0.25 μg/ml～110 μg/ml for Vitamin A（r0.9994）.The average recovery was 97.3% and RSD was 1.2%.Conclusion： The method that determination of Vitamin A in duplicate diets by HPLC was simple,sensitive,rapid and accurate.