产ESBLs肺炎克雷伯菌的RAPD基因分型研究

A molecular epidemiology study on Extended-Spectrum Beta-Lactamases producing Klebsiella pneumoniae with randomly amplified polymorphic DNA

目的:建立肺炎克雷伯菌的基因扩增多态性DNA分型方法,了解产ESBLs肺炎克雷伯菌院内感染的分子流行情况。方法:收集临床各病区送检的产ESBLs肺炎克雷伯菌感染标本29份。采用随机扩增DNA多态性方法对产ES-BLs肺炎克雷伯菌进行基因分型。利用Phylip3.67聚类分析软件对RAPD扩增条带结果进行聚类分析。结果:RAPD扩增的指纹图谱清晰,带型稳定,多态性丰富。29株不同来源的菌株可大致分为三种亲缘关系。结论:随机扩增多态性DNA技术可以用于产ESBLs肺炎克雷伯菌基因多态性分型研究;产ESBLs肺炎克雷伯菌基因多态性分布可能与菌株的来源有关。

Objective：To genotype clinical Klebsiella pneumoniae isolates by randomly amplified polymorphic DNA（RAPD） method,study molecular epidemiology of extended-spectrum beta-lactamases（ESBLs） producing Klebsiella pneumoniae infection.Methods： 29 ESBLs Klebsiella pneumoniae isolates with corresponding patients information.All the isolates were genotyped by RAPD.Cluster analysis was performed using Neighbor Joining method by Phylip3.67 soft.Results： Primer was suitable for fingerprinting analysis and was found to generate the reproducible fingerprints,yielding well-resolved banding patterns.Generally,the isolates can be divided into three clusters.Conclusion： RAPD fingerprinting can be used to genotype Klebsiella pneumoniae;the RAPD patterns of ESBLs Klebsiella pneumoniae may be related to origins of fungi;it seems the RAPD patterns also related to drug resistance.