蛋白芯片法检测幽门螺杆菌CagA、Hsp60和Ure的应用

The application of protein chip test for Helicobacter Pylori CangA,Hsp60 and Ure

目的:应用不同方法调查公共场所从业人员HP感染状况,探讨公共场所服务人员HP感染率及存在的传染隐患。方法:应用福建三强生物化工有限公司提供的幽门螺杆菌快速诊断试剂盒进行Hp的尿素酶快速诊断试验,同时应用幽门螺杆菌蛋白芯片(南京大渊生物技术工程有限责任公司),对142份血样进行CagA、Ure和Hsp60抗体测定。结果:142份公共场所从业人员血样检测HP尿素酶抗体阳性率分别为68.30%(福建三强)和62.68%(南京大渊);CagA毒力株感染者68份,占HP检测人数的47.89%;未检测到Hsp60抗体阳性血样。结论:HP检测可及时提示感染和传染,是否将HP检测纳入公共场所从业人员健康体检。

Objective：Use different methods for public employees of HP infection status and explore public service personnel HP infection rates and existence.Methods： Application of Fujian sanqiang biological chemical Co.,LTD.Offers helicobacter pylori rapid diagnostic kit for Hp′s urease rapid diagnostic tests also applied helicobacter pylori protein microarray（nanjing bigyuan biotechnology engineering limited liability company） determination for 142 blood sample of CagA,Ure,and Hsp60 antibody.Results： 142 blood sample Hp urea antibodies positive tate separately is 68.30%（Fujian sanqiang） and 62.68%（Nanjing dayuan）;CagA antibody positive 68 and infected rate is 47.89%.Hsp60 antibody positive samples have been not detected.Conclusion： HP testing can timely clew infection and whether Hp testing use in public places personnel healthy check-up.